Biosynthesis in vitro of bacillamide intermediate-heterocyclic AlaCys thiazole by heterologous expression of nonribosomal peptide synthetase (NRPS).

Fengli Zhang, Nayila Mulati,Yukun Wang,Yingxin Li,Sanqiang Gong,Loganathan Karthik,Wei Sun,Zhiyong Li

Journal of Biotechnology（2019）

引用 3|浏览11

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摘要

•Two modules of nonribosomal peptide synthetase were heterologous expressed and purified.•In vitro set up a biosynthetic reaction system of nonribosomal peptide.•Bacillamide C intermediate- heterocyclic AlaCysthiazole was formed.

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关键词

Bacillus atrophaeus,Bacillamides,Heterologous expression,Nonribosomal peptide synthetase (NRPS),Thiazole

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