Laccase activity measurement by FTIR spectral fingerprinting.

•Laccase oxidation reaction can be monitored using FTIR spectral profiling.•FTIR coupled to PARAFAC can be used to assess and quantify laccase activity.•Laccases can be distinguished on the basis of their origin by assessing the oxidation reaction pattern.•FTIR combined with multiway analysis can create a reference system for comparison of laccase action across large data sets.