Akazamicin, a cytotoxic aromatic polyketide from marine-derived Nonomuraea sp

In our screening program on marine-derived actinomycetes, Nonomuraea sp. AKA32 isolated from deep-sea water collected from a depth of 800 m in Sagami Bay, Japan was found to produce compounds cytotoxic to cancer cells. Activity-guided purification led to the isolation of a new aromatic polyketide, akazamicin ( 1 ), along with two known compounds, actinofuranone C ( 2 ) and N -formylanthranilic acid ( 3 ). Structures of these compounds were elucidated through the interpretation of NMR and MS spectroscopic data. Compounds 1 , 2 , and 3 displayed cytotoxicity against murine B16 melanoma cell line with the IC 50 value of 1.7, 1.2, and 25 μM, respectively.