Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy.
Methods in molecular biology (Clifton, N.J.)(2019)
Abstract
Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.
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Key words
Hepatitis,Imaging,Microscopy,Super-resolution,Virus
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