[Protective effects of deep sea water against subacute alcoholic liver injury].

OBJECTIVE:To study the protective effects of two deep sea water( DSW)samples with different ion concentration against sub-acute alcoholic liver injury. METHODS:A total of 72 male KM mice( 18-22 g) were randomly assigned into six groups, i. e. the control group, model group, DSW-A1 fold concentration group( A1), DSW-A5 folds concentration group( A5), DSW-A 10 folds concentration group( A10), DSW-B5 fold concentration group( B). The corresponding DSW solution was ingested freely by each DSW group, and deionized water was ingested freely by the other two groups. The total administration duration was 30 consecutive days. Groups were given 50% ethanol solution( 0. 10 m L/10( g·d)) by ig since the 15 th day except the control group. After administration period, detect serum aspartate aminotransferase( AST), alanine aminotransferase( ALT), total bilirubin( T-BIL), total cholesterol( TC), triglyceride( TG) and low density lipoprotein( LDL), liver TC, TG, GSH, malonaldehyde( MDA), glutathione/glutathione oxidized( GSH/GSSG), superoxide dismutase( SOD), glutathione peroxidase( GSH-Px), catalase( CAT), xanthine oxidase( XOD). Livers were also further detected by microscopic examinations. RESULTS:Compared with the control group, the model group had serious liver steatosis. The histological score and serum T-BIL were significantly increased( P < 0. 01), serum TC, LDL, AST and liver MDA were significantly increased( P < 0. 05), liver GSH-Px was significantly decreased( P < 0. 01). Compared with the model group, in A1 group, the serum T-BIL was significantly decreased( P < 0. 05), liver GSH-Px( P < 0. 01) and XOD( P < 0. 05) were significantly increased. In A5 group, serum T-BIL, liver MDA and pathologic changes were significantly decreased( P < 0. 01), serum TG and TC were significantly decreased( P <0. 05), liver GSH( P < 0. 01), GSH/GSSG( P < 0. 05), GSH-Px( P < 0. 01) and XOD( P < 0. 01) were significantly increased. In A10 group, serum LDL and AST were significantly decreased( P < 0. 05), serum TG, TC, T-BIL, liver MDA and the pathologic changes were significantly decreased( P < 0. 01), liver GSH/GSSG( P < 0. 05) and GSHPx( P < 0. 01) were significantly increased. In B group, serum TC and liver MDA were significantly decreased( P < 0. 05), serum TG, T-BIL, AST and liver pathologic changes were significantly decreased( P < 0. 01), liver XOD( P < 0. 05) and GSH-Px( P< 0. 01) were significantly increased. CONCLUSION:Middle and high concentration DSWA and DSW-B have protective effects against sub-acute alcoholic liver injury by decreasing serum lipid level, and improving the ability of antioxidation.