五产区牡丹根际及根内放线菌研究
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica(2018)
Abstract
采用高通量测序技术研究了牡丹皮五产区采收期牡丹根际及根内放线菌多样性.测序获得的129 954条优质序列分为1 754个可操作分类单元,其中从根际土壤中获得的1 311个OTU检出放线菌4纲、4目、27科、67属,Ilumatobacter等33属在五产区根际土壤中均有分布,Longispora等3属仅分布于道地产区,Mycobacterium,Nocardioides,Streptomyces为根际放线菌优势属.根中获得的443个OTU检出放线菌3纲、3目、24科,50属,Cryptosporangium等13属在五产区根中均有分布,Planosporangium,Luteococcus仅分布于道地产区,Nocardioides为根内放线菌优势属.Alpha多样性分析得到毫州、南陵、铜陵产区根际放线菌多样性指数(Chaol,Shannon指数)大于菏泽、洛阳.PCoA分析表明铜陵、南陵产区根际放线菌以及铜陵和洛阳产区根内放线菌组成较为接近.Heatmap显示铜陵、南陵、亳州产区根际放线菌系统发育相似,铜陵、洛阳、南陵产区根内放线菌相似.研究表明牡丹根际、根内放线菌种群组成多样,道地产区根际放线菌系统发育相似性较高.
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Key words
Dao-di features,Paeonia suffruticosa,actinomycetes,diversity,high-throughput sequencing technique
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