Disruption of peroxisome proliferator–activated receptor γ coactivator (PGC)-1α reverts key features of the neoplastic phenotype of glioma cells
Journal of Biological Chemistry(2019)
Abstract
The peroxisome proliferator-activated receptor coactivator (PGC)-1 is a master regulator of mitochondrial biogenesis and controls metabolism by coordinating transcriptional events. Here, we interrogated whether PGC-1 is involved in tumor growth and the metabolic flexibility of glioblastoma cells. PGC-1 was expressed in a subset of established glioma cell lines and primary glioblastoma cell cultures. Furthermore, a higher PGC-1 expression was associated with an adverse outcome in the TCGA glioblastoma dataset. Suppression of PGC-1 expression by shRNA in the PGC-1-positive U343MG glioblastoma line suppressed mitochondrial gene expression, reduced mitochondrial membrane potential, and diminished oxygen as well as glucose consumption, and lactate production. Compatible with the known PGC-1 functions in reactive oxygen species (ROS) metabolism, glioblastoma cells deficient in PGC-1 displayed ROS accumulation, had reduced RNA levels of proteins involved in ROS detoxification, and were more susceptible to death induction by H2O2 compared with control cells. PGC-1sh cells also had impaired proliferation and migration rates in vitro and displayed less stem cell characteristics. Complementary effects were observed in PGC-1-low LNT-229 cells engineered to overexpress PGC-1. In an in vivo xenograft experiment, tumors formed by U343MG PGC-1sh glioblastoma cells grew much slower than control tumors and were less invasive. Interestingly, the PGC-1 knockdown conferred protection against hypoxia-induced cell death, probably as a result of less active anabolic pathways, and this effect was associated with reduced epidermal growth factor expression and mammalian target of rapamycin signaling. In summary, PGC-1 modifies the neoplastic phenotype of glioblastoma cells toward more aggressive behavior and therefore makes PGC-1 a potential target for anti-glioblastoma therapies.
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Key words
glioblastoma,hypoxia,reactive oxygen species (ROS),transcription coactivator,tumor metabolism,peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) (PPARGC1A)
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