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Alkaline phosphatase levels of murine pre-osteoblastic cells on anodized and annealed titanium surfaces.

Sinem Yeniyol,John Lawrence Ricci

EUROPEAN ORAL RESEARCH（2018）

Cited 3|Views13
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Abstract
Purpose This study aimed to evaluate the initial adhesion morphology and alkaline phosphatase (ALP) activity of murine pre-osteoblastic MC3T3-E1 cells cultured on anatase/rutile mixed-phase TiO2 thin films with photocatalytical activity with previously confirmed antibacterial properties. Materials and methods Anatase/rutile mixed-phase TiO2 thin films fabricated by anodization and annealing of cpTi were used to culture MC3T3-E1 cells to evaluate the initial cellular adhesion morphology and ALP activity in vitro. Results Compared with MC3T3-E1 cells cultured on cpTi substrates and the control group, cells cultured on anatase/rutile mixed-phase TiO2 thin films exhibited similar ALP levels after cell culture day 9. Conclusion Anodizing and annealing processes fabricate multifunctional surfaces on cpTi with improved osteogenic properties for implants.
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Key words
Titanium dioxide,surface roughness,crystal phase,alkaline phosphatase,pre-osteoblastic cell
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