Application of Daphnia magna for Nanoecotoxicity Study.

Daphnia magna (D. magna), an aquatic invertebrate, is the most commonly used test organism in ecotoxicological studies on manufactured nanomaterials (MNs). Although standard protocols for undertaking acute and chronic toxicity tests of dissolved chemicals with D. magna have been endorsed by several national and international organizations, comprehensive guidance on aquatic toxicology testing of MNs with D. magna are in their infancy. Here we describe the acute and chronic toxicity methods with D. magna as test organism for nanoecotoxicity study. These methods may provide reference for the next step toward developing prescriptive aquatic toxicity standard tests for MNs with D. magna.