The mating system of the Eucalyptus canker pathogen Chrysoporthe austroafricana and closely related species.

•Genomes of C. austroafricana, C. deuterocubensis and C. cubensis were sequenced.•Heterothallism and homothallism predicted in Chrysoporthe species.•C. austroafricana, C. deuterocubensis and C. cubensis have unique MAT loci.•C. austroafricanaMAT1-2 locus contains a truncated MAT1-1-1 gene.•LTRs predicted in the MAT loci of C. deuterocubensis and C. cubensis.•First characterization of the MAT locus among the described Chrysoporthe species.