A light-initiated chemiluminescent assay for rapid quantitation of allergen-specific IgG 4 in clinical samples.

•An increase in allergen specific IgG4 levels indicates acquisition of tolerance.•LICA is a homogeneous bead-based serological assay.•An immunoquantitation kit for allergen sIgG4 is currently lacking in China.•The LICA for allergen sIgG4 exhibited satisfactory performance.•Levels of Gal d 1, Gal d 2, and Gal d 3 sIgG4 are different among allergic, atopic, and healthy subjects.