Quantification of xanthine- and uric acid-related compounds in urine using a "dilute-and-shoot" technique coupling ultra-high-performance liquid chromatography and high-resolution Orbitrap mass spectrometry.
Journal of Chromatography B(2017)
Abstract
•Analysis of twelve uric acid- and xanthine- related compounds in urine.•Caffeine, theobromine, theophylline and their metabolites are target analytes.•Different compounds related to renal lithiasis can be also analyzed.•No sample pretreatment is needed. Dilute-and-shoot technique is used.•Short analysis time and low LLOQ.
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Key words
Liquid chromatography,Orbitrap,High resolution mass spectrometry,Methylxantine analysis,Uric acid
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