Bacillus Endozanthoxylicus Sp Nov., An Endophytic Bacterium Isolated From Zanthoxylum Bungeanum Maxim Leaves

A Gram-stain-positive, rod-shaped, motile bacterium, designated as 1404(T), was isolated from leaves of Chinese red pepper (Huajiao) (Zanthoxylum bungeanum Maxim) collected from Gansu, north-west China. Spores were not observed under a range of conditions. Strain 1404(T) was observed to grow at 15-45 degrees C and pH 6.0-10.0 and in presence of 0-5% (w/v) NaCl concentration. The cell wall of strain 1404(T) was found to contain meso-diaminopimelic acid, and the predominant respiratory quinone was identified as MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as well as three unidentified polar lipids. The major fatty acids profile of strain 1404(T) consisted of iso-C-15:0 (25.6 %), anteiso-C-15:0 (18.4 %) and iso-C-14:0 (12.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1404(T) was affiliated to the genus Bacillus and was closely related to Bacillus oryzisoli 1DS3-10(T), Bacillus benzoevorans DSM 5391(T) and Bacillus circulans DSM 11(T) with sequence similarity of 98.3, 98.2 and 96.9 %, respectively. The G+C content of the genomic DNA was determined to be 39.4 mol%. DNA-DNA hybridization values indicated that relatedness between strain 1404(T) and the type strains of closely related species of the genus Bacillus was below 41 %. Therefore, on the basis of the data from the polyphasic taxonomic study presented, strain 1404(T) represents a novel species of the genus Bacillus, for which the name proposed is Bacillus endozanthoxylicus sp. nov. The type strain is 1404(T) (= CCTCC AB 2017021(T) = KCTC 33827(T)).