Tellurite And Tellurate Reduction By The Aerobic Anoxygenic Phototroph Erythromonas Ursincola, Strain Kr99 Is Carried Out By A Novel Membrane Associated Enzyme
MICROORGANISMS(2017)
摘要
Erythromonas ursincola, strain KR99 isolated from a freshwater thermal spring of Kamchatka Island in Russia, resists and reduces very high levels of toxic tellurite under aerobic conditions. Reduction is carried out by a constitutively expressed membrane associated enzyme, which was purified and characterized. The tellurite reductase has a molecular weight of 117 kDa, and is comprised of two subunits (62 and 55 kDa) in a 1:1 ratio. Optimal activity occurs at pH 7.0 and 28 degrees C. Tellurite reduction has a V-max of 5.15 mu mol/min/mg protein and a K-m of 3.36 mM. The enzyme can also reduce tellurate with a V-max and K-m of 1.08 mu mol/min/mg protein and 1.44 mM, respectively. This is the first purified membrane associated Te oxyanion reductase.
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关键词
tellurite,tellurate,tellurium,reduction,aerobic anoxygenic phototroph
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