Promoter, transgene, and cell line effects in the transfection of mammalian cells using PDMAEMA-based nano-stars.

Alexander Raup,Valérie Jérôme,Ruth Freitag,Christopher V Synatschke,Axel H E Müller

Biotechnology reports (Amsterdam, Netherlands)（2016）

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摘要

•4 cell lines, 4 promoters, and 3 gene products were studied, i.e. 48 combinations.•Distinct cell line depended effects were observed.•Jurkat cell results tended to differ from that obtained with the other cells.•Co-transfection works well in CHO cells, but fails in up to 80% of Jurkat cells.•High transfection efficiency in CHO and HEK cells is maintained in spite of pDNA dilution.

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关键词

Fluorescent reporter protein,Co-expression,Non-viral gene delivery,Mammalian cell,Poly(2-dimethylamino) ethyl methacrylate

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