Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions.
Biotechnology Reports(2016)
摘要
•Single domain antibody—rhizavidin fusion bioconjugated biotin coated quantum dots.•Provided facile and effective method to orient antibodies on QD surface.•Accomplished improved production of His-tagged rhizavidin (RZh) in E. coli.•Demonstrated utility of RZh as a replacement for tetrameric biotin binders.
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关键词
Single domain antibodies,Rhizavidin,Quantum dots,Surface plasmon resonance
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