Conjugation of biotin-coated luminescent quantum dots with single domain antibody-rhizavidin fusions.

Jinny L Liu,Scott A Walper,Kendrick B Turner,Audrey Brozozog Lee,Igor L Medintz,Kimihiro Susumu,Eunkeu Oh,Dan Zabetakis,Ellen R Goldman,George P Anderson

Biotechnology Reports（2016）

引用 15|浏览7

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摘要

•Single domain antibody—rhizavidin fusion bioconjugated biotin coated quantum dots.•Provided facile and effective method to orient antibodies on QD surface.•Accomplished improved production of His-tagged rhizavidin (RZh) in E. coli.•Demonstrated utility of RZh as a replacement for tetrameric biotin binders.

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关键词

Single domain antibodies,Rhizavidin,Quantum dots,Surface plasmon resonance

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