Structural modeling of protein–RNA complexes using crosslinking of segmentally isotope-labeled RNA and MS/MS

Georg Dorn,Alexander Leitner,Julien Boudet,Sébastien Campagne,Christine von Schroetter,Ahmed Moursy,Ruedi Aebersold,Frédéric H-T Allain

NATURE METHODS（2017）

引用 36|浏览20

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摘要

mass spectrometry–based method to pinpoint UV-induced crosslinks in ribonucleoprotein complexes at protein residue and RNA nucleotide resolution provides key structural information for integrative modeling.

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关键词

Mass spectrometry,Protein structure predictions,RNA-binding proteins,Solution-state NMR,Life Sciences,general,Biological Techniques,Biological Microscopy,Biomedical Engineering/Biotechnology,Bioinformatics,Proteomics

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