A novel model to assess lamellar signaling relevant to preferential weight bearing in the horse.

•A novel model to study lamellar signaling events during preferential weight bearing is presented.•Lamellar signaling events related to hypoxia and inflammation were assessed.•Lamellar hypoxia inducible factor-1a was increased in the supporting limb relative to the contralateral hind limb.•No differences in lamellar inflammatory signaling were present between the supporting limb and hind limbs.