Fournierella Massiliensis Gen. Nov., Sp Nov., A New Human-Associated Member Of The Family Ruminococcaceae

An anaerobic bacterium, strain AT2(T), was isolated from the fresh stool sample of a healthy French man using the culturomics approach. The 16S rRNA gene sequence analysis showed that strain AT2(T) had 95.2% nucleotide sequence similarity with Gemmiger formicilis ATCC 27749(T), the phylogenetically closest species with standing in nomenclature. Cells are Gram-stain-negative, catalase- and oxidase-negative, obligately anaerobic, non-motile, non-spore-forming, rod-shaped, and the bacilli were mesothermophilic. The major fatty acids were C-16 : 0 (43.8 %) and C-18 : 1n9 (20 %). The DNA G+C content of the strain based on its genome sequence was 56.8 mol%. Based on the phenotypic, biochemical and phylogenetic analysis, we propose the creation of the genus Fournierella gen. nov., which contains strain AT2(T) (=CSUR P2014(T)=DSM 100451(T)) as the type strain of the type species Fournierella massiliensis gen. nov., sp. nov.