Identification Of Antibiotic Resistance Genes In The Multidrug-Resistant Acinetobacter Baumannii Strain, Mdr-Shh02, Using Whole-Genome Sequencing

Hualiang Wang, Jinghua Wang,Peijuan Yu, Ping Ge,Yanqun Jiang,Rong Xu,Rong Chen, Xuejie Liu

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE(2017)

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摘要
This study aimed to investigate antibiotic resistance genes in the multidrug-resistant (MDR) Acinetobacter baumannii (A. baumanii) strain, MDR-SHH02, using whole-genome sequencing (WGS). The antibiotic resistance of MDR-SHH02 isolated from a patient with breast cancer to 19 types of antibiotics was determined using the Kirby-Bauer method. WGS of MDR-SHH02 was then performed. Following quality control and transcriptome assembly, functional annotation of genes was conducted, and the phylogenetic tree of MDR-SHH02, along with another 5 A. baumanii species and 2 Acinetobacter species, was constructed using PHYLIP 3.695 and FigTree v1.4.2. Furthermore, pathogenicity islands (PAIs) were predicted by the pathogenicity island database. Potential antibiotic resistance genes in MDR-SHH02 were predicted based on the information in the Antibiotic Resistance Genes Database (ARDB). MDR-SHH02 was found to be resistant to all of the tested antibiotics..The total draft genome length of MDR-SHHO2 was 4,003,808 bp. There were 74.25% of coding sequences to be annotated into 21 of the Clusters of Orthologous Groups (COGs) of protein terms, such as 'transcription' and `amino acid transport and metabolism'. Furthermore, there were 45 PAIs homologous to the sequence MDRSHH02000806. Additionally, a total of 12 gene sequences in MDR-SHH02 were highly similar to the sequences of antibiotic resistance genes in ARDB, including genes encoding aminoglycoside-modifying enzymes [e.g., aac(3)-Ia, ant(2 '')-Ia, aph33ib and aph(3')-Ia], beta-lactamase genes (b12b_tem and bl2b_teml), sulfonamide resistant dihydropteroate synthase genes (sull and sul2), catb3 and tetb. These results suggest that numerous genes mediate resistance to various antibiotics in MDR-SHH02, and provide a clinical guidance for the personalized therapy of A. baumannii-infected patients.
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关键词
Acinetobacter baumannii, antibiotic resistance gene, whole-genome sequencing
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