MBTH: A novel approach to rapid, spectrophotometric quantitation of total algal carbohydrates.
Analytical Biochemistry(2017)
摘要
A high-throughput and robust application of the 3-methyl-2-benzothiazolinone hydrazone (MBTH) method was developed for carbohydrate determination in microalgae. The traditional phenol-sulfuric acid method to quantify carbohydrates is strongly affected by algal biochemical components and exhibits a highly variable response to microalgal monosaccharides. We present a novel use of the MBTH method to accurately quantify carbohydrates in hydrolyzate after acid hydrolysis of algal biomass, without a need for neutralization. The MBTH method demonstrated consistent and sensitive quantitation of algae-specific monosaccharides down to 5 μg mL−1 without interference from other algae acidic hydrolyzate components.
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关键词
Algae,Carbohydrates,Spectrophotometric,Phenol-sulfuric acid,MBTH
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