Catechol-O-Methyltransferase Promoter Hypomethylation Is Associated With The Risk Of Coronary Heart Disease

Catechol-O-methyltransferase (COMT) gene variation is known to be associated with the risk of acute coronary events. The purpose of the present study was to investigate the contribution of COMT promoter methylation towards the risk of coronary heart disease (CHD). COMT methylation was evaluated in 48 CHD cases and 48 well-matched non-CHD controls using bisulfite pyrosequencing technology. The results demonstrated that CHD cases had a significantly lower level of methylation at COMT CpG3 sites compared with the controls (33.77 +/- 5.71 vs. 36.42 +/- 5.00%; P= 0.018). Further analysis, according to gender, showed that CpG3 methylation was associated with CHD in males (P= 0.038) but not in females (P= 0.253), suggesting that there is a gender disparity in the association between COMT methylation and CHD. In conclusion, it was determined that COMT CpG3 hypomethylation is associated with an increased risk of CHD in males.