Development of a 45kpsi ultrahigh pressure liquid chromatography instrument for gradient separations of peptides using long microcapillary columns and sub-2μm particles.

Kaitlin M Grinias,Justin M Godinho,Edward G Franklin,Jordan T Stobaugh,James W Jorgenson

Journal of Chromatography A（2016）

引用 33|浏览2

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摘要

•Development of a constant pressure UHPLC gradient system.•Development of a fully-automated constant pressure UHPLC gradient system.•Peak capacities exceeding 800 for peptide separations.•Gradient separations at 45kpsi.

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关键词

UHPLC,Capillary chromatography,LC–MS,Proteomics,Peptides

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