Protective effects of parecoxib on rat primary astrocytes from oxidative stress induced by hydrogen peroxide
JOURNAL OF ZHEJIANG UNIVERSITY-SCIENCE B(2016)
Abstract
Objective To investigate the protective effects of parecoxib from oxidative stress induced by hydrogen peroxide (H 2 O 2 ) in rat astrocytes in vitro. Methods All experiments included 4 groups: (1) negative control (NC) group, without any treatment; (2) H 2 O 2 treatment group, 100 µmol/L H 2 O 2 treatment for 24 h; (3) and (4) parecoxib pretreatment groups, 80 and 160 µmol/L parecoxib treatment for 24 h, respectively, and then treated with 100 µmol/L H 2 O 2 . Several indices were investigated, and the expressions of Bax, Bcl-2, and brain-derived neurotrophic factor (BDNF) were quantified. Results Compared to the NC group, exposure to H 2 O 2 resulted in significant morphological changes, which could be reversed by pretreatment of parecoxib. In addition, H 2 O 2 treatment led to loss of viability (P=0.026) and increased intracellular reactive oxygen species (ROS) levels ( P <0.001), and induced apoptosis ( P <0.01) in the primary astrocytes relative to the NC group. However, in the parecoxib pretreatment groups, all the above changes reversed significantly ( P <0.05) as compared to the H 2 O 2 treatment group, and were nearly unchanged when compared to the NC group. Mechanical investigation showed that dysregulated Bax, Bcl-2, and BDNF could be implicated in these changes. Conclusions Our results indicated that parecoxib provided a protective effect from oxidative stress induced by exposure to H 2 O 2 .
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Key words
Parecoxib,Primary astrocyte,Hydrogen peroxide (H2O2),Brain-derived neurotrophic factor (BDNF),Bax,Bcl-2
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