Development and optimization of a multiplex lateral flow immunoassay for the simultaneous determination of three mycotoxins in corn, rice and peanut

•A multiplex lateral flow immunoassay was developed for the determination of three mycotoxins.•The preparation of antibody-gold nanoparticle conjugate was optimized.•The detection limits were far below the regulatory limits set by the European Union.•The assay procedure can be completed within 15min.