Biophysical and biochemical studies of a major endoglucanase secreted by Xanthomonas campestris pv. campestris.

Flávio Rodolfo Rosseto,Livia Regina Manzine,Mario de Oliveira Neto,Igor Polikarpov

Enzyme and Microbial Technology（2016）

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摘要

•A major cellulolytic enzyme XccCel5A, secreted by the bacterium Xanthomonas campestris pv. campestris was expressed and purified.•Biophysical, biochemical and enzymatic characterization of XccCel5A was conducted.•Low-resolution SAXS molecular envelope of the endoglucanase in solution was determined.

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关键词

Xanthomonas campestris,Endoglucanases,Hydrolysis,SAXS

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