A high-throughput hydrophilic interaction liquid chromatography coupled with a charged aerosol detector method to assess trisulfides in IgG1 monoclonal antibodies using tris(2-carboxyethyl)phosphine reaction products: Tris(2-carboxyethyl)phosphine-oxide and tris(2-carboxyethyl)phosphine-sulfide.

Christopher Cornell,Amish Karanjit,Yan Chen,Fredric Jacobson

Journal of Chromatography A（2016）

引用 6|浏览3

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摘要

•A chromatographic HILICCAD method to measure levels of mAb trisulfide modifications.•Comparable results to the alternative method—peptide map with LC/MS detection.•Method applicable to cysteine-linked ADC production and cell culture process development.

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关键词

Trisulfides,Hydrophilic interaction chromatography,Charged aerosol detector,Tris(2-carboxyethyl)phosphine (TCEP),Tris(2-carboxyethyl)phosphine-oxide (TCEP[O]),Tris(2-carboxyethyl)phosphine-sulfide (TCEP[S]),Antibody-drug conjugates,Monoclonal antibodies

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