Chromatin Immunoprecipitation-Sequencing (ChIP-seq) for Mapping of Estrogen Receptor-Chromatin Interactions in Breast Cancer.
ESTROGEN RECEPTORS: METHODS AND PROTOCOLS(2016)
摘要
Chromatin immunoprecipitation-sequencing (ChIP-Seq) is a powerful tool which combines the established method of ChIP with next-generation sequencing (NGS) to determine DNA-binding sites of a protein of interest on a genome-wide level, importantly, allowing for de novo discovery of binding events. Here we describe ChIP-seq using the well-established example of estrogen receptor-α mapping in the MCF7 breast cancer cell line.
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关键词
Chromatin,Chromatin immunoprecipitation,Enhancers,Estrogen receptor,Next-generation sequencing,Promoters
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