Assessment of Maternal Cell Contamination in Prenatal Samples by Quantitative Fluorescent PCR (QF-PCR).
Methods in molecular biology (Clifton, N.J.)（2019）
Abstract
When biopsying a fetal tissue like chorionic villi or amniotic fluid, there is a chance of getting some maternal material that could contaminate the fetal specimen and might lead to a misdiagnosis. Thus, all prenatal samples should be subjected to testing for maternal cell contamination. This is done using quantitative fluorescent PCR (QF-PCR) of short tandem repeat (STR) markers.
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Key words
Genotyping,Maternal cell contamination,Microsatellite markers,Prenatal diagnosis,Sample identity,Short tandem repeats
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