Liquid chromatography-mass spectrometry-based urinary metabolomics study on a rat model of simulated microgravity-induced depression.

•Rat model of depression was induced by 14-d simulated microgravity (SMG) treatment.•Urinary metabolic profiles of SMG-treated and control rats showed distinct separation.•Of the 22 identified biomarkers, 15 were verified by LC-QQQ-MS/MS.•SMG-induced depression is related to metabolism of certain amino acids.•SMG-induced depression is related to perturbations in energy metabolism.