Dissociation Of F-19 And Fluorescence Signal Upon Cellular Uptake Of Dual-Contrast Perfluorocarbon Nanoemulsions

ObjectivePerfluorocarbon nanoemulsions (PFCs) tagged with fluorescence dyes have been intensively used to confirm the in vivo F-19 magnetic resonance imaging (MRI) localization of PFCs by post mortem histology or flow cytometry. However, only limited data are available on tagged PFCs and the potential dissociation of fluorescence and F-19 label after cellular uptake over time.Materials and methodsPFCs were coupled to rhodamine (Rho) or carboxyfluorescein (Cfl) and their fate was analyzed after in vitro uptake by J774, RAW and CHO cells by flow cytometry and F-19 MRI. In separate in vivo experiments, the dual-labelled emulsions were intravenously applied into mice and their distribution was monitored in spleen and liver over 24h. In a final step, time course of fluorescence and F-19 signals from injected emulsions were tracked in a local inflammation model making use of a subcutaneous matrigel depot doped with LPS (lipopolysaccharide).ResultsInternalization of fluorescence-labelled PFCs was associated with a substantial whitening over 24h in all macrophage cell lines while the F-19 signal remained stable over time. In all experiments, (PFCs)-P-Cfl were more susceptible to bleaching than (PFCs)-P-Rho. After intravenous injection of (PFCs)-P-Rho, the fluorescence signal in spleen and liver peaked after 30min and 2h, respectively, followed by a successive decrease over 24h, whereas the F-19 signal continuously increased during this observation period. Similar results were found in the matrigel/LPS model, where we observed increasing F-19 signals in the inflammatory hot spot over time while the fluorescence signal of immune cells isolated from the matrigel depot 24h after its implantation was only marginally elevated over background levels. This resulted in a massive underestimation of the true PFC deposition in the reticuloendothelial system and at inflammatory hot spots.ConclusionCellular uptake of fluorescently tagged PFCs leads to a dissociation of the fluorescence and the F-19 label signal over time, which critically impacts on interpretation of long-term experiments validated by histology or flow cytometry.