Internal sample process control improves cultivation-independent quantification of thermotolerant Campylobacter
Food Microbiology(2019)
摘要
Quantification of Campylobacter is challenging and one major reason is the fact that bacteria lose cultivability due to cold or oxygen stress during storage at retail. Alternative live/dead discriminatory qPCR currently lacks standardization and might overestimate live cells in the presence of dead cells.
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关键词
VBNC,qPCR,Propidium monoazide,Oxidative stress,Food safety
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