Assay validation of hair androgens across the menstrual cycle.

•Androgen concentrations are significantly associated in hair and saliva samples.•Hair androgen levels are stable within and across hair samples.•Female saliva androgens, primarily DHEA, are variable across menstrual cycle.•Hair assay can be used as a good measure for basal hormone levels.•EIA hair assay method is validated with correspondence of hair and saliva samples.