Paracandidimonas Caeni Sp. Nov., Isolated From Sludge

A beige-pigmented, Gram-stain-negative, aerobic, non-motile, rod-shaped bacterium, named 24(T), was isolated from sludge of a pesticide manufacturing factory in Nantong, Jiangsu Province, China. 16S rRNA gene sequence analysis revealed that strain 24(T) shared highest similarity with Parapusillimonas granuli Ch07(T) (98.20 %), followed by Candidimonas nitroreducens SC-089(T) (98.07 %) and Paracandidimonas soli IMT-305(T) (98.03 %). Phylogenetic trees showed that strain 24(T) formed a distinct clade with Paracandidimonas soli IMT-305(T). The results of DNA-DNA hybridization tests showed that reassociation values were less than 45 % with respect to these closely related type strains. Strain 24(T) contained Q-8 and putrescine as the major respiratory quinone and polyamine, respectively. The main cellular fatty acids were summed feature 3 (C-16 : 1 omega 7c/C-16 : 1 omega 6c), summed feature 2 (iso-C-16 : 1 I/C-14 0 3-OH/C-12 : 0 aldehyde), summed feature 8 (C-18 : 1 omega 7c/C-18 : 1 omega 6c) and C-12 : 0. The polar lipid profile included phosphatidylmethylethanolamin, phosphatidylethanolamine, phosphatidylglycerol, one unidentified phospholipid and one unidentified aminolipid. The G+C content was 56.83 mol%. Combined data from phenotypic, phylogenetic and DNA-DNA relatedness studies demonstrated that strain 24(T) represents a novel species of the genus Paracandidimonas , for which the name Paracandidimonas caeni sp. nov. is proposed. The type strain is 24(T) (=CCTCC AB 2018057(T)=KACC 19692(T)).