Cellular tension encodes local Src-dependent differential β 1 and β 3 integrin mobility.
MOLECULAR BIOLOGY OF THE CELL(2019)
Abstract
Integrins are transmembrane receptors that have a pivotal role in mechanotransduction processes by connecting the extracellular matrix to the cytoskeleton. Although it is well established that integrin activation/inhibition cycles are due to highly dynamic interactions, whether integrin mobility depends on local tension and cytoskeletal organization remains surprisingly unclear. Using an original approach combining micropatterning on glass substrates to induce standardized local mechanical constraints within a single cell with temporal image correlation spectroscopy, we measured the mechanosensitive response of integrin mobility at the whole cell level and in adhesion sites under different mechanical constraints. Contrary to beta 1 integrins, high tension increases beta 3 integrin residence time in adhesive regions. Chimeric integrins and structure-function studies revealed that the ability of beta 3 integrins to specifically sense local tensional organization is mostly encoded by its cytoplasmic domain and is regulated by tuning the affinity of its NPXY domains through phosphorylation by Src family kinases.
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Key words
integrin mobility,cellular tension encodes,src-dependent
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