SRSF1 modulates PTPMT1 alternative splicing to regulate lung cancer cell radioresistance.

•The splicing factor SRSF1 is identified to regulate cancer cell resistance to IR.•Depleted SRSF1 switches PTPMT1 splicing to sensitize cancer cells to radiation.•PTPMT1 splicing switch induces AMPK phosphorylation to increase radiosensitivity.•PTPMT1B is markedly reduced in cancers, indicating potentials in cancer treatment.