Production of a bioflocculant from Klebsiella sp. OS-1 using brewery wastewater as a source.

This study evaluated the potential of bioflocculant production from a Klebsiella strain using brewery wastewater as nutrients. The bioflocculant named OS-1B produced by Klebsiella sp. OS-1 exhibited a good flocculating activity to kaolin clay suspension (around 95%), when the diluted brewery wastewater with 7.2 mg/L total nitrogen and 1013 mg/L CODCr was used as a nitrogen source. Glucose (15 g/L) is the most favorable carbon source for Klebsiella sp. OS-1 in bioflocculant production from brewery wastewater. The yielded bioflocculant is pH tolerant and thermally stable, suggesting its good industrial potential. OS-1B mainly comprises polysaccharide (69.4%) and protein (24.5%). Fourier-transform infrared spectra indicate the presence of hydroxyl, carboxyl, esters and amino groups in the bioflocculant molecules. Combined with the results of zeta potential measurements, bridging is suggested as the main flocculation mechanism for OS-1B flocculation with kaolin. Overall, brewery wastewater can be used as a substrate to produce bioflocculants.