The Effects Of Silencing The Her2 Gene On Proliferation And Angiogenesis Of Meningioma Cells In Vivo And In Vitro

Despite the molecular mechanisms of meningioma having been elucidated, the curative effects of current treatments for invasive and malignant meningiomas have been unsatisfactory. Our previous study found that HER2 protein was overexpressed in human meningiomas. However, only a few studies regarding the correlation between meningiomas and HER2 have been reported. The present study aimed to investigate the influence of silencing the Her2 gene on the proliferation and angiogenesis of human malignant meningioma cells. Human malignant meningioma cells were transfected successfully by special shRNA. After lentivirus infection, mRNA and protein levels of Her2 in the shRNA group were significantly reduced. Cell viability began to decrease at 72 h and was most strongly inhibited at 96 h, as measured by CCK-8 assay. Protein levels of Ki-67 and VEGF in the Her2-sh group were significantly lower than in the control and mock groups. After injecting tumor cells into nude mice, the tumor volume was significantly lower in the Her2-sh group, and protein levels of Ki-67, VEGF and CD34 were significantly lower in Her2-sh group than in the control and mock groups. The results demonstrated that silencing Her2 may inhibit the proliferation and angiogenesis of human meningioma cells.