miR4673 improves fitness profile of neoplastic cells by induction of autophagy

Therapeutic resistance of neoplasms is mainly attributed to gradual evolution of mutational profile 1 . Here, we demonstrate a microRNA-mediated mechanism that effectively improves fitness of SKBR3 mammary carcinoma cells by cytoplasmic reprogramming. The reprogramming is triggered by endogenous miR4673 transcribed from notch-1 locus. The miRNA downregulates cdk-18, a cyclin-dependent kinase that regulates M-G1 transition in cycling cells 2 , 3 . Suppression of cdk-18 triggers mitophagy and autophagy. Due to high autophagic flux, oestrogen receptor-1 + /progesterone receptor + /p53 + (Esr1 + /Pr + /p53 + ) SKBR3 cells are coerced into an Esr1 − /Pr low /p53 − profile. Increased mitophagy in combination with proteasomal degradation of p53 transiently arrests the cycling cells at G0 and enhances radio-resistance of the SKBR3 population. These findings highlight the impact on cancer therapy of non-encoded neoplastic resistance, arising as a consequence of miRNA-mediated autophagic reprogramming that uncouples phenotype and genotype.