Imaging SNAP-29 in Drosophila.
Methods in molecular biology (Clifton, N.J.)(2019)
Abstract
SNAP-29 is expressed throughout the life cycle of fruit fly and exhibits wide tissue distribution patterns. Unlike other SNAP-25-like proteins (i.e., SNAP-25, SNAP-23/24, and SNAP-47) which primarily support exocytosis at the plasma membrane, SNAP-29 regulates various intracellular trafficking events, by partnering with proteins active in both exocytosis and endocytosis. Here we describe the protocol to localize SNAP-29 in early embryos, imaginal discs from third instar larva, and immortalized S2 cells via immunofluorescence microscopy.
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Key words
Drosophila,SNAP-29,Ubisnap,usnp
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