Streptomyces monticola sp. nov., a novel actinomycete isolated from soil

A novel actinobacterium, designated strain NEAU-GS4 T , was isolated from soil collected from Mount Song and characterised using a polyphasic approach. 16S rRNA gene sequence similarity studies showed that strain NEAU-GS4 T belongs to the genus Streptomyces , being closely related to Streptomyces spectabilis JCM 4308 T (98.8%), Streptomyces sclerotialus DSM 43032 T (98.3%) and Streptomyces lasiicapitis 3H-HV17(2) T (98.0%). A multilocus sequence analysis based on five house-keeping genes ( atp D, gyr B, rpo B, rec A and trp B) also indicated that strain NEAU-GS4 T should be assigned to the genus Streptomyces . The major menaquinones were identified as MK-9(H 6 ), MK-9(H 8 ) and MK-9(H 4 ). The polar lipid profile was found to contain diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositolmannosides and an unidentified phospholipid. The major fatty acids were identified as anteiso-C 15:0 , iso-C 16:0 and anteiso-C 17:0 . Moreover, DNA–DNA hybridization results and some phenotypic characteristics indicated that strain NEAU-GS4 T can be clearly differentiated from its closely related species of the genus Streptomyces . Therefore, it is concluded that strain NEAU-GS4 T represents a novel species of the genus of Streptomyces , for which the name Streptomyces monticola sp. nov. is proposed. The type stain is NEAU-GS4 T (=CGMCC 4.7467 T = DSM 105116 T ).