Single clip: An improvement of the filament-perforation mouse subarachnoid haemorrhage model.
BRAIN INJURY(2019)
摘要
Objective: The endovascular filament-perforation model turned out to become the most popular one for the reproduction of prominent pathophysiological features observed after human subarachnoid haemorrhage (SAH). However, few studies have considered methods that may minimize surgically induced injury. This study described an improved and simplified surgical procedure in which a single clip is placed at the external carotid artery (ECA).Method: Male C57BL/6 mice were given either a classic endovascular filament SAH model, improved endovascular filament SAH model or sham injury. Multiple strategies, including MRI with T-2-weighted imaging and F-18-FDG PET/CT scanning, were performed to compare the improved and classic SAH models.Results: The new method of filament model resulted a typical pathophysiological progress of early brain injury (EBI), including cerebral oedema, blood brain barrier (BBB) disruption, neuronal apoptosis and microglia activation. The improved SAH model is characterized by a shorter operation time (15.650.64min vs. 21.75 +/- 0.94min), reduced surgically induced injury (decreased F-18-FDG standardized uptake values (SUV): 1.7 +/- 0.07 vs. 2.02 +/- 0.11), and stable cerebral perfusion before SAH.Conclusions: The improved surgical technique appears to be a feasible tool for experimental and translational studies of SAH.
更多查看译文
关键词
Subarachnoid haemorrhage,early brain injury,filament model,translational research
AI 理解论文
溯源树
样例
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要