LPS promote Osteosarcoma invasion and migration through TLR4/HOTAIR.

OBJECTIVES:Osteosarcoma is one of common malignant tumors worldwide in the metaphysis of teenagers. The role of lncRNAs in Osteosarcoma has become an emerging area of research. MATERIALS AND METHODS:Cell migration and invasion were analyzed in Osteosarcoma cell following knockdown or overexpression by transfection with small interfering RNA (siRNA) or treated with LPS. Western blotting and Real-time RT-PCR methods were used to analyze the effects of LPS on EMT. RESULTS:We discovered that LPS could regulate cell migration and invasion and promote EMT. At the same time, LPS could regulate the expression of TLR4 and HOTAIR. In addition, knockdown of the expression of TLR4 partially reverses the promotion of cell invasion induced by LPS. CONCLUSIONS:Our results indicated that LPS coordinate the Osteosarcoma through TLR4/HOTAIR.