Conditional reprogramming and long-term expansion of normal and tumor cells from human biospecimens
NATURE PROTOCOLS(2017)
摘要
This protocol demonstrates how to establish primary epithelial cell cultures in vitro from healthy human tissue and human cancer samples using ROCK inhibitor and irradiated feeder cells.
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关键词
Cancer models,Cell culture,Reprogramming,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry
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