Bhargavaea changchunensis sp. nov. isolated from soil in China

A Gram-staining-positive, aerobic, rod-shaped (201802YP6 T ) bacteria was isolated from soil, Northeast of China. Growth occurred at 10–40 °C (optimum 25–30 °C), at pH 6.0–8.0 (optimum 7.0) and at 0–2% NaCl. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbors of strain 201802YP6 T were identified as Bhargavaea cecembensis DSE10 T (99.52%), Bhargavaea beijingensis ge10 T (99.45%), Bhargavaea indica KJW98 T (99.45%), Bhargavaea ullalensis ZMA19 T (98.81%), and Bhargavaea ginsengi ge14 T (98.76%). Levels of similarity among strain 201802YP6 T and other Bhargavaea species were lower than 98.76%. GyrB amino acid sequence-based analysis supported the phylogenetic position and also distinguished strain 201802YP6 T from the other species of the genus Bhargavaea . DNA–DNA hybridization values between strain 201802YP6 T and B. cecembensis, B. beijingensis, B. indica, B. ullalensis, B. ginsengi were 43.5%, 43%, 32.5%, 30.5% and 20.4%, respectively. The DNA G + C content of strain 201802YP6 T was 51.23 mol%. The average nucleotide identity (ANI) of the draft genome was 87.04% to B. cecembensis DSE10 T . The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipids, phosphatidylethanolamine, and phosphatidyllipid. The predominant menaquinone was MK-8. The major fatty acids were iso-C 15:0 (39.91%), anteiso-C 15:0 (28.86%), anteiso-C 17:0 (6.30%) and C 16:0 (6.13%). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain 201802YP6 T represents a novel species of the genus Bhargavaea , for which the name Bhargavaea changchunensis sp. nov. is proposed. The type strain is 201802YP6 T (= CGMCC 1.13508 T = KCTC 33975 T ).