Influence Of Nd:Yag Laser On The Penetration Of A Bioceramic Root Canal Sealer Into Dentinal Tubules: A Confocal Analysis

ObjectiveThe aim of this in vitro study, is to evaluate the penetration of a bioceramic root canal sealer into dentinal tubules at 3 mm and 5 mm from the apex after Nd:YAG laser irradiation.MethodsForty freshly extracted human mandibular premolars were prepared using Reciproc (R) and irrigated with 17% ethylenediaminetetraacetic acid (EDTA). Teeth were divided into 4 groups: group 1, obturated with control sealer (AH Plus (R)); group 2, obturated with bioceramic sealer (Endosequence BC Sealer (R)); group 3, Nd: YAG laser + control sealer (AH Plus (R)); and group 4, Nd: YAG laser + bioceramic sealer (Endosequence BC Sealer (R)). The samples were transversely sectioned 3 mm and 5 mm from the apex and examined using confocal laser scanning microscopy. Two parameters were measured: 1) sealer penetration into dentinal tubules of the root canal and 2) sealer penetration into the perimeter of the root canal walls.ResultsPenetration analysis showed that bioceramic sealer had a higher penetration at depths of 3 and 5 mm than that of the control sealer, regardless of laser use (p < 0.05). Perimeter analysis showed that there was no difference between both sealers at a depth of 3 mm (p < 0.05), regardless of laser use. At a depth of 5 mm, bioceramic sealer and laser showed a greater perimeter of penetration (p < 0.05) than the control sealer.ConclusionThe use of Nd:YAG laser did not compromise the penetration of bioceramic sealer into dentinal tubules of root canals at 3 mm and 5 mm from the apex.