Dissemination of bla OXA-370 is mediated by IncX plasmids and the Tn6435 transposon

In Enterobacteriaceae , the bla OXA-48 -like genes have been identified on plasmids in different regions of the world. The OXA-370 is a plasmid-encoded OXA- 48-like enzyme reported in two distinct regions of Brazil. Recently, we demonstrate that the bla OXA-370 gene is disseminated among several Enterobacteriaceae species and clones, indicating a high potential for dissemination. In this work, we described for the first time the complete nucleotide sequence of six plasmids harboring the bla OXA-370 gene. Complete DNA sequencing using the Illumina platform and annotation of the plasmids showed that they belonged to incompatibility groups IncX and had in average 70 kbp. The bla OXA-370 gene is located in a composite transposon containing four genes encoding transposases, named Tn6435. In this study, highly similar plasmids were detected in different Enterobacteriaceae genera.