Enhanced photorespiration in transgenic rice over-expressing maize C4 phosphoenolpyruvate carboxylase gene contributes to alleviating low nitrogen stress
Plant Physiology and Biochemistry(2018)
摘要
The objective of this study was to reveal the physiological and molecular mechanisms of low-nitrogen (N) tolerance in transgenic plant lines containing C4 phosphoenolpyruvate carboxylase (C4-PEPC) gene. The transgenic rice lines only over-expressing the maize C4-PEPC) (PC) and their untransformed wild type, Kitaake (WT), were used in this study. At different N levels, the dry weight, total N content, carbon and N levels, photorespiration-related enzymatic activities, gene expression levels and photorespiration-related product accumulations were measured, as were the transgenic lines' agronomic traits. The PC line, having lower total N and higher soluble sugar contents, was more tolerant to low-N stress than WT, which was consistent with its higher PEPC and lower N-assimilation-related enzyme activity levels. The photosynthetic parameters, enzymatic activity levels, transcripts and products related to photorespiration in PC were also greater than in WT under low-N conditions. This study showed that increased carbon levels in transgenic rice lines overexpressing C4-PEPC could help regulate the photorespiratory pathway under low-N conditions, conferring low-N tolerance and a higher grain yield per plant.
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关键词
Low-nitrogen stress,Phosphoenolpyruvate carboxylase,Photorespiration,Transgenic C4-PEPC-Overexpressing rice (Oryza sativa L.)
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