Predictive kinetic optimisation of hydrophilic interaction chromatography × reversed phase liquid chromatography separations: Experimental verification and application to phenolic analysis.

•A Pareto optimality approach for method development in HILIC × RP-LC is reported.•Dilution and/or splitting of 1D flow used to overcome mobile phase incompatibility.•Predicted optimal conditions applied to the HILIC × RP-LC separation of procyanidins.•Good agreement found between the predicted and experimental performance.•Derived optimal conditions offer increased separation power and lower dilution.