Effect of Progranulin on Migration and Invasion of Human Colon Cancer Cells.

Objective: To investigate the effects of progranulin on migration and invasion of human colon cancer cells. Study Design: An experimental study. Place and Duration of Study: Department of Histology and Embryology, College of Basic Medical Sciences, Jilin University, Changchun, China, from October 2015 to July 2017. Methodology: Cell transfection was used to obtain progranulin knocked down/overexpressing cells. MTT and soft agar assay were applied to investigate the cell growth. Migration and invasion were detected using transwell assay. Results: Tumor growth, migration and invasion decreased in progranulin knocked down cells and increased in progranulin overexpressing cells. The decrease was recovered when exogenous progranulin was added. Progranulin-induced migration and invasion were inhibited by neamine. Conclusion: Progranulin has a direct effect on tumor growth, migration, and invasion of human colon cancer cells. It may serve as a potential therapeutic target for human colon cancer.